Coding

Part:BBa_K944003

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


cioA gene related to cyanide insensitivity


cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 434
    Illegal XhoI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
    Illegal NgoMIV site found at 988
    Illegal NgoMIV site found at 1129
    Illegal NgoMIV site found at 1371
    Illegal NgoMIV site found at 1393
    Illegal AgeI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1336
    Illegal SapI.rc site found at 1411


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Parameters
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